α-Amylase Activity Assay Kit - E-BC-K007-M

α-Amylase Activity Assay Kit - E-BC-K007-M

  • €150,00
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Detection principle

The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance, which is inactivated by the thermolabile nature of β-amylase, and then the enzyme activity of α-amylase is determined.

Performance characteristics

Sample type animal and plant tissue
Sensitivity 0.0097 U/mL
Detection range 0.0097-0.3474 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 40 min
Precision Average inter-assay CV: 3.900%Average intra-assay CV: 3%
Other instruments required Micropipettor, Multi-channel pipettor, Incubator, Vortex mixer, Centrifuge
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.97-34.74 U/g tissue).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

1% Epipremnum aureum tissue homogenate


1% Green pepper tissue homogenate


1% Corn grain tissue homogenate


1% Daucus carota tissue homogenate


Note: The diluent is double distilled water.