8-OHdG8-Hydroxydeoxyguanosine ELISA Kit - E-EL-0028
Product Details
Properties
| Assay type | Competitive-ELISA |
| Format | 96T/48T |
| Assay time | 2.0h |
| Detection range | 1.56-100 ng/mL |
| Sensitivity | 0.94 ng/mL |
| Sample type &Sample volume | serum, plasma and other biological fluids; 50μL |
| Specificity | This kit recognizes 8-OHdG in samples. No significant cross-reactivity or interference between 8-OHdG and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Application | This ELISA kit applies to the in vitro quantitative determination of 8-OHdG concentrations in serum, plasma and other biological fluids. |
Test Principle
Kit components & Storage
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips 48T: 8 wells ×6 strips |
-20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial |
|
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 48T: 1 vial, 60 μL |
|
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
| HRP Conjugate Diluent | 1 vial, 14 mL | |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
Technical Data
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 8-OHdG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 8-OHdG were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 4.79 | 10.11 | 46.98 | 4.48 | 9.61 | 48.89 |
| Standard deviation | 0.27 | 0.58 | 1.69 | 0.27 | 0.55 | 1.91 |
| CV (%) | 5.66 | 5.69 | 3.60 | 5.95 | 5.70 | 3.90 |
Recovery
The recovery of 8-OHdG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 86-100 | 91 |
| EDTA plasma(n=8) | 91-104 | 98 |
| Cell culture media(n=8) | 93-106 | 98 |
Linearity
Samples were spiked with high concentrations of 8-OHdG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Target Information
| Research Area | Metabolism, Cardiovascular, Immunology, Cancer |
Assay Procedures
 |
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
 |
2. Aspirate and wash the plate for 3 times |
 |
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
 |
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
 |
5. Add 50μL Stop Solution |
 |
6. Read the plate at 450nm immediately. Calculation of the results |
Citations
-
NATURE (2020) IF: 42.779
DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25PMID: 32528174
Sample: DNA -
Redox Biology (2021) IF: 11.799
Biomarkers of nucleic acid oxidation – a summary state-of-the-artPMID: 33579665