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Catalase CAT Activity Assay Kit - E-BC-K031-M

Catalase CAT Activity Assay Kit - E-BC-K031-M

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Detection principle

The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.

Performance characteristics

Synonyms CAT
Sample type Serum,plasma,cells,cell culture supernatant,tissue
Sensitivity 1.12 U/mL
Detection range 1.12 -150 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 40 min
Precision Average inter-assay CV: 7.700%Average intra-assay CV: 3.900%
Other instruments required Micropipettor, Vortex Mixer, Incubator
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

The activity of CAT increased significantly after treated with melatonin in 5 μM of cadmium. (*P<0.05)

D Bartolini et al investigate the mechanism of melatonin protecting porcine pre-pubertal Sertoli cell from cadmium through regulating Nrf2 acivity. Catalase (CAT) activity of porcine pre-pubertal Sertoli cell was determined using CAT activity assay kit (E-BC-K031-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.12 -150 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

293T supernatant

1

10% Rat heart tissue homogenization

50-100

10% Rat liver tissue homogenization

100-200

10% Rat spleen tissue homogenization

50-100

Mouse serum

1

10% Epipremnum aureum tissue homogenization

1-2

10% Rat lung tissue homogenization

50-100

10% Rat kidney tissue homogenization

50-100

10% Rat brain tissue homogenization

20-50

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


Citations


  1. ACS Nano (2020) IF: 14.588
    Nonrecurring Circuit Nanozymatic Enhancement of Hypoxic Pancreatic Cancer Phototherapy Using Speckled Ru–Te Hollow Nanorods

    DOI: 10.1021/acsnano.9b09974
    Sample: RuTeNRs
  2. JOURNAL OF PINEAL RESEARCH (2022) IF: 13.007
    Melatonin modulates Nrf2 activity to protect porcine pre-pubertal Sertoli cells from the abnormal H2O2 generation and reductive stress effects of cadmium.

    DOI: 10.1111/jpi.12806
    Sample: Sertoli cell (SCs)