Catalase CAT Activity Assay Kit - E-BC-K031-S
Detection principle
The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.
Performance characteristics
| Synonyms | CAT |
| Sample type | Serum,plasma,cells,tissue |
| Sensitivity | 0.27 U/mL |
| Detection range | 0.27-155.4 U/mL |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 70 min |
| Precision | Average inter-assay CV: 5.100%Average intra-assay CV: 3.100% |
| Other instruments required | Micropipettor, Vortex mixer, Incubator or Water-bath |
| Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.27-155.4 U/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
The volume of sample |
|
10% Rat liver tissue homogenization |
25-50 |
50 μL |
|
10% Rat kidney tissue homogenization |
10-25 |
50 μL |
|
10% Rat brain tissue homogenization |
5-10 |
50 μL |
|
Human serum |
1 |
100 μL |
|
293T supernatant |
1 |
100 μL |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Citations
-
JOURNAL OF PINEAL RESEARCH (2022) IF: 13.007
Melatonin modulates Nrf2 activity to protect porcine pre-pubertal Sertoli cells from the abnormal H2O2 generation and reductive stress effects of cadmium.DOI: 10.1111/jpi.12806
Sample: Sertoli cell (SCs) -
Journal of Advanced Research (2022) IF: 10.479
Cadmium accumulation regulated by a rice heavy-metal importer is harmful for host plant and leaf bacteriaPMID: 35640876
Sample: leaf