Catalase CAT Activity Assay Kit - E-BC-K031-S

Catalase CAT Activity Assay Kit - E-BC-K031-S

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Detection principle

The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.

Performance characteristics

Synonyms CAT
Sample type Serum,plasma,cells,tissue
Sensitivity 0.27 U/mL
Detection range 0.27-155.4 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 70 min
Precision Average inter-assay CV: 5.100%Average intra-assay CV: 3.100%
Other instruments required Micropipettor, Vortex mixer, Incubator or Water-bath
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.27-155.4 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

The volume of sample

10% Rat liver tissue homogenization


50 μL

10% Rat kidney tissue homogenization


50 μL

10% Rat brain tissue homogenization


50 μL

Human serum


100 μL

293T supernatant


100 μL

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


    Melatonin modulates Nrf2 activity to protect porcine pre-pubertal Sertoli cells from the abnormal H2O2 generation and reductive stress effects of cadmium.

    DOI: 10.1111/jpi.12806

    Sample: Sertoli cell (SCs)
  2. Journal of Advanced Research (2022) IF: 10.479
    Cadmium accumulation regulated by a rice heavy-metal importer is harmful for host plant and leaf bacteria

    DOI: 10.1016/j.jare.2022.05.010

    PMID: 35640876

    Sample: leaf