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CuZn-SOD/Mn-SOD Activity Assay Kit - E-BC-K022-S

CuZn-SOD/Mn-SOD Activity Assay Kit - E-BC-K022-S

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Detection principle

Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.

 

 

Performance characteristics

Synonyms CuZn-SOD,T-SOD
Sample type Serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity 2.03 U/mL
Detection range 2.03-155 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 100 min
Precision Average inter-assay CV: 7.200%Average intra-assay CV: 4%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl)
Storage Reagent 4: -20℃, others: 2-8℃
Valid period 12 months

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

The volume added to the reaction (μL)

Mouse serum

3-5

20-30

Human serum

1

30-40

Rat serum

3-5

20-30

Rat plasma

1-2

20-30

Human hydrothorax

1

30-50

Human urine

1

30-50

10% Mouse liver tissue homogenate

50-80

20-30

10% Mouse brain tissue homogenate

8-12

20-30

10% Mouse kidney tissue homogenate

10-20

20-30

HepG2 cells (3.27 mgprot/mL)

5-10

20-30

Note: The diluent is normal saline (0.9% NaCl).


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