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D-Lactic Acid LA Colorimetric Assay Kit - E-BC-K002-M

D-Lactic Acid LA Colorimetric Assay Kit - E-BC-K002-M

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etection principle

Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D-lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.#N/A

Performance characteristics

Synonyms D-LA
Sample type Serum, plasma, animal tissue, saliva sample
Sensitivity 0.06 mmol/L
Detection range 0.06-8.0 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 20 min
Precision Average inter-assay CV: 7.700%Average intra-assay CV: 3.800%
Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

Plasma D-LA level was significantly increased in VDN group, and reversed in SP treated group. (****P<0.0001)

Jianlong Yan et al investigates the beneficial effect of the short-chain fatty acid in intestinal microbiota remodeling. Plasma D-Lactate of rat was determined using D-lactate colorimetric assay kit (E-BC-K002-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.06-8.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human plasma

1

Human serum

1

Rat serum

1

Rat plasma

1

Mouse serum

1

Rabbit serum

1

10% Rat kidney tissue homogenate

2-3

10% Mouse brain tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).



  1. Microbiome (2022) IF: 16.837
    Beneficial effect of the short-chain fatty acid propionate on vascular calcification through intestinal microbiota remodelling

    DOI: 10.1186/s40168-022-01390-0
    Sample: plasma
  2. International Journal of Nanomedicine (2023) IF: 7.033
    Nano Parthenolide Improves Intestinal Barrier Function of Sepsis by Inhibiting Apoptosis and ROS via 5-HTR2A

    DOI: 10.2147/IJN.S394544
    Sample: blood