Free Fatty Acids FFA Fluorometric Assay Kit - E-BC-F039

Detection principle

Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.

Performance characteristics

Images

The concentration of FFA in rat plasma was significantly increased in T2DM and PPG treated group, and decreased with ALA treatment.

G J Dugbartey et al investigate liver injury induced by diabetes. Free Fatty Acid (FFA) concentration in rat plasma was determined using FFA fluorometric assay kit (E-BC-F039).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.58-20 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Note: The diluent is reagent 1.

Ralated product

Citations

1. Nature Communications (2022) IF: 17.694

   Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease

   DOI: 10.1038/s41467-022-35481-1
   PMID: 36572670	Sample: serum

2. CELL PROLIFERATION (2022) IF: 8.7550

   Hypothalamic Hnscr regulates glucose balance by mediating central inflammation and insulin signal

   DOI: 10.1111/cpr.13332
   	Sample: liver