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Lactic Acid LA Assay Kit Serum/Tissue samples - E-BC-K044-S

Lactic Acid LA Assay Kit Serum/Tissue samples - E-BC-K044-S

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Detection principle

Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm.

Performance characteristics

Sample type Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity 0.05 mmol/L
Detection range 0.05-6.0 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 60 min
Precision Average inter-assay CV: 1.900%Average intra-assay CV: 1.100%
Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

DOX administration significantly increased hippocampal lactic acid content (*P<0.05 vs. Control group), AML co-treatment significantly lowered hippocampal lactic acid content to near control levels. AML alone treated rats showed no significant difference when compared to control group. (#P<0.05 and ###P<0.001 vs. DOX group)

A E Ali investigate the antitumor effects of AML and its ability to mitigate cancer resistance to DOX therapy. L-Lactate (L-LA) of rat hippocampal was determined using L-lactate colorimetric assay kit (E-BC-K044-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-6.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

4-8

10% Mouse muscle tissue homogenization

2-4

10% Mouse liver tissue homogenization

1

Rat serum

4-8

HePG2 cells homogenization (1.388 gprot/L)

4-8

HepG2 supernatant

2-4

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


Citations


  1. Cell Communication and Signaling (2022) IF: 7.525
    Inhibition of AMPK/PFKFB3 mediated glycolysis synergizes with penfluridol to suppress gallbladder cancer growth

    DOI: 10.1186/s12964-022-00882-8

    PMID: 35842652

    		 Sample: SGC-996 cell,EH-GB1 cell,GBC-SD cell
  2. CANCER SCIENCE (2022) IF: 6.518
    circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1

    DOI: 10.1111/cas.15351
    Sample: AGS cell,HGC-27 cell