Cart 0
Malondialdehyde MDA Assay Kit TBA method - E-BC-K025-M

Malondialdehyde MDA Assay Kit TBA method - E-BC-K025-M

Shipping calculated at checkout.

Detection principle

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.

Performance characteristics

Synonyms MDA
Sample type Serum,plasma,animal tissue
Sensitivity 1.13 μmol/L
Detection range 2.92-40 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 65 min
Precision Average inter-assay CV: 7.200%Average intra-assay CV: 4.100%
Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge, Magnetic Stirrers
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol
Storage 2-8℃
Valid period 12 months

Images

The level of MDA was significantly lower (P<0.001) in NPCoQ10 groups and even lower in N-NPCoQ10 group.

Z Liu et al found that the usage of N-NPCoQ10 nano-particle can help for transforming the antioxidants which could ameliorate the renal I-R injury in mice. Malondialdehyde (MDA) of rat serum was determined using MDA colorimetric assay kit (E-BC-K025-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (2.92-40 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

Rat serum

1

Rat plasma

1

Mouse serum

1

Mouse plasma

1

10% Rat heart tissue homogenate

1

10% Rat liver tissue homogenate

1

10% Rat spleen tissue homogenate

1

10% Rat lung tissue homogenate

1

10% Rat kidney tissue homogenate

1

10% Rat brain tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).



Citations


  1. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.1010
    miR-495–3p regulates sphingolipid metabolic reprogramming to induce Sphk1/ceramide mediated mitophagy and apoptosis in NSCLC

    DOI: 10.1016/j.freeradbiomed.2022.07.001
    Sample: A549 cell,H1299 cell
  2. Journal of Neuroinflammation (2021) IF: 8.3219
    Sirtuin 3 protects against anesthesia/surgery-induced cognitive decline in aged mice by suppressing hippocampal neuroinflammation

    DOI: 10.1186/s12974-021-02089-z

    PMID: 33541361

    		 Sample: Tissue homogenate(Hippocampal homogenate)