Malondialdehyde MDA Assay Kit TBA method - E-BC-K025-S
Detection principle
MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Performance characteristics
| Synonyms | MDA |
| Sample type | Serum,plasma,animal tissue |
| Sensitivity | 0.38 nmol/mL |
| Detection range | 0.38-133.33 nmol/mL |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 90 min |
| Precision | Average inter-assay CV: 8%Average intra-assay CV: 4.900% |
| Other instruments required | Micropipettor, Vortex mixer, Incubator, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol |
| Storage | 2-8℃ |
| Valid period | 12 months |
Images
The level of MDA was significantly increased (*P<0.005) in cells treating with the nanoreactor.
XX Yang et al investigates the function of a nanoreactor that can induce ferroptosis of tumor cells by releasing DHA and iron. Malondialdehyde (MDA) of Lewis lung cancer cells was determined using MDA colorimetric assay kit (E-BC-K025-S).
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (0.38-133.33 nmol/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
Human serum |
1 |
|
Human plasma |
1 |
|
Rat serum |
1 |
|
Rat plasma |
1 |
|
Mouse serum |
1 |
|
Mouse plasma |
1 |
|
10% Rat heart tissue homogenate |
1 |
|
10% Rat liver tissue homogenate |
1 |
|
10% Rat spleen tissue homogenate |
1 |
|
10% Rat lung tissue homogenate |
1 |
|
10% Rat kidney tissue homogenate |
1 |
|
10% Rat brain tissue homogenate |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
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JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 10.435
A nanoreactor boosts chemodynamic therapy and ferroptosis for synergistic cancer therapy using molecular amplifier dihydroartemisininPMID: 35568865
Sample: Lewis cell -
CELLULAR & MOLECULAR BIOLOGY LETTERS (2023) IF: 8.702
Formyl peptide receptor 2 activation by mitochondrial formyl peptides stimulates the neutrophil proinflammatory response via the ERK pathway and exacerbates ischemia–reperfusion injurySample: Kidney tissue