Malondialdehyde MDA Colorimetric Assay Kit - E-BC-K027-S

Malondialdehyde MDA Colorimetric Assay Kit - E-BC-K027-S

  • €220,00
    Unit price per 
Shipping calculated at checkout.


Detection principle

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.

 

Performance characteristics

Synonyms MDA
Sample type Plant tissue
Sensitivity 0.17 nmol/mL
Detection range 0.17-120 nmol/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 90 min
Precision Average inter-assay CV: 6.100%Average intra-assay CV: 2.200%
Other instruments required Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator
Other reagents required Absolute ethanol
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.17-120 nmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Epipremnum aureum tissue homogenate

1

10% Green pepper tissue homogenate

1

10% Eggplant tissue homogenate

1

10% Bitter gourd tissue homogenate

1

Note: The diluent is reagent 5 working solution.


Citations


  1. Annals of Translational Medicine (2022) IF: 3.932
    Levosimendan protects from sepsis-inducing cardiac dysfunction by suppressing inflammation, oxidative stress and regulating cardiac mitophagy via the PINK-1-Parkin pathway in mice

    DOI: 10.21037/atm-22-483

    PMID: 35280364

    Sample: myocardium
  2. BIOLOGICAL RHYTHM RESEARCH (2022) IF: 1.219
    Impact of photoperiods on the specific activities of immune and antioxidant enzymes in different tissues of Dybowski’s frog (Rana dybowskii)

    DOI: 10.1080/09291016.2022.2043588

    Sample: serum,liver,skin