Malondialdehyde MDA Colorimetric Assay Kit - E-BC-K027-S

Malondialdehyde MDA Colorimetric Assay Kit - E-BC-K027-S

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Detection principle

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.


Performance characteristics

Synonyms MDA
Sample type Plant tissue
Sensitivity 0.17 nmol/mL
Detection range 0.17-120 nmol/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 90 min
Precision Average inter-assay CV: 6.100%Average intra-assay CV: 2.200%
Other instruments required Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator
Other reagents required Absolute ethanol
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.17-120 nmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Epipremnum aureum tissue homogenate


10% Green pepper tissue homogenate


10% Eggplant tissue homogenate


10% Bitter gourd tissue homogenate


Note: The diluent is reagent 5 working solution.


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