miRcute miRNA Isolation Kit (ref 4992860)
Store at room temperature (15-25℃ )
Buffer MZ should be stored at 2-8℃ away from light
DescriptionThe miRcute miRNA Isolation Kit is a miRNA extraction and isolation kit based on the adsorption column method. It can efficiently extract small RNAs such as miRNA, siRNA, snRNA, etc., which are 20-200 nt in length, and can also extract total RNA. The efficient lysis buffer can easily lyse various tissues and cells. The adsorption column uses a special silicon matrix membrane packing, which greatly enhances its ability to adsorb RNA, especially small RNA (<200 nt).
Required ReagentsEthanol, chloroform
■ Efficient and accurate separation of small RNA fragments at the sizes of 20-200 nt.
■ Can be applied on small fragment RNA extraction as well as total RNA extraction.
■ Easy to operate, all operations can be completed in 1 hour.
■ High purity, the extracted RNA is free of DNA and protein contamination, facilitating the analysis of downstream experiments.
■ Northern Blot analysis.
■ Real-Time PCR.
■ Microarray analysis.
The Ct value of the miRNA extracted from 30 mg mouse liver using miRcute miRNA Isolation Kit was smaller than that of traditional isopropanol precipitation method.
The absorption peak curve of miRNA extracted from 30 mg mouse liver samples using miRcute miRNA Isolation Kit. This figure shows that the miRNA extracted by miRcute miRNA Isolation Kit is more pure than the traditional isopropanol precipitation method.
■ Ding J, Wen Q, Huo Z, Nie H, Qin Y, Yan X. Identification of shell-color-related microRNAs in the Manila clam Ruditapes philippinarum using high-throughput sequencing of small RNA transcriptomes. Sci Rep. 2021 Apr 13;11(1):8044.
■ Jing Zhang et al. Co-Expression of miR155 or LSD1 shRNA Increases the Anti-Tumor Functions of CD19 CAR-T Cells. Frontiers in Immunology 2022.