Plant Genomic DNA Kit (ref 4992201)
StorageRoom temperature (15-25℃)
DescriptionThe Plant Genomic DNA Kit provides a fast, simple, and cost-effective genomic DNA extraction method for routine molecular biology laboratory applications. It’s especially suitable for genomic DNA purification from powder or polysaccharide/polyphenolics rich plants. The kit is based on the silica membrane technology and a unique buffer to eliminate polysaccharide, polyphenolics, and enzyme inhibitors. Plant Genomic DNA Kit is ready-to-use to purify the genomic DNA from a wide variety of plants. The purified DNA is suitable for PCR, restriction enzyme digestion and Southern blot.
Required ReagentsLiquid nitrogen, Ethanol, Phenol-chloroform, RNaseA (optional)
■ Simple and fast: The purified gDNA could be obtained within 1 hour.
■ High purity and excellent quality: The high pure genomic DNA could be used directly in downstream experiments such as PCR amplification, restriction enzyme digestion and Southern blot.
■ Suitable for various plant tissues.
■ Particularly suitable for polysaccaride/polyphenol-rich plant tissues.
■ Particularly suitable for plant powders.
DNA Extraction Yield From Various Plant Tissues
Genomic DNA isolated from 100 mg leaves of different plants using Plant Genomic DNA Kit. 3 μl of 100 μl eluates were loaded per lane.
1: Fresh tomato leaves;
2: Fresh cotton leaves;
3: Fresh tea leaves;
4: Fresh strawberry leaves.
The concentration of the agarose gel was 1%. The electrophoresis was performed under 6 V/cm for 20 min.
M: λDNA/Hind III Marker
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