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Reduced Glutathione GSH Assay Kit - E-BC-K030-M

Reduced Glutathione GSH Assay Kit - E-BC-K030-M

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Detection principle

Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly.

 

Performance characteristics

Synonyms GSH
Sample type Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity 2 μmol/L
Detection range 2-100 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 25 min
Precision Average inter-assay CV: 3.200%Average intra-assay CV: 1.900%
Other instruments required Micropipettor, Vortex mixer
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

Significant differences can be observed comparing with P group. (*P<0.05, **P<m0.01, ***p<0.001)<="" p="">

G G Liou et al found that N-acetyl cysteine can reduce propacetamol-induced hepatotoxicity with low dose, but induce with high dose. Reduced glutathione level of mouse liver tissue was determined using GSH colorimetric assay kit (E-BC-K030-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2-100 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

10% Mouse brain tissue homogenization

1

10% Mouse liver tissue homogenization

1

Hela cell  homogenization（0.999 mgprot/mL）

1

Rat serum

1

Rat plasma

1

Mouse serum

1

10% Carrot tissue homogenization

1

293T supernatant

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


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