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Reduced glutathione GSH Assay Kit - E-BC-K030-S

Reduced glutathione GSH Assay Kit - E-BC-K030-S

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Detection principle

Reduced glutathione (GSH) can react with dithionitrobenzoic acid (DTNB) to produce thio-nitrobenzoic acid and glutathione disulfide. Nitromercaptobenzoic acid is a yellow compound which has the maximum absorption peak at 420 nm. The GSH content can be calculated by measuring the OD value at 420 nm.

Performance characteristics

Synonyms GSH
Sample type Serum,plasma,tissue,cells
Sensitivity 0.26 mg GSH/L
Detection range 0.26-122.8 mgGSH/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 50 min
Precision Average inter-assay CV: 2.400%Average intra-assay CV: 1.800%
Other instruments required Micropipettor, Vortex mixer
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

The level of GSH was significantly changed (*P<0.01) in D100, D200 and Co2+ groups.

D Wang et al investigates the acute toxicity and oxidative damage caused by cobalt-based framework. Reduced glutathione level of Photobacterium phosphoreum T3 was determined using GSH colorimetric assay kit (E-BC-K030-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of pre-experiment and the detection range ( 0.26-122.8 mg GSH/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Mouse serum

1

10% Mouse brain tissue homogenization

1

10% Rat liver tissue homogenization

1

Human plasma

1

Rat plasma

1

10% Carrot tissue homogenization

1

293T supernatant

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


Citations


  1. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 10.435
    A nanoreactor boosts chemodynamic therapy and ferroptosis for synergistic cancer therapy using molecular amplifier dihydroartemisinin

    DOI: 10.1186/s12951-022-01455-0

    PMID: 35568865

    		 Sample: Lewis cell
  2. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Mesenchymal Stem Cell-Derived Exosomes Ameliorate Delayed Neurocognitive Recovery in Aged Mice by Inhibiting Hippocampus Ferroptosis via Activating SIRT1/Nrf2/HO-1 Signaling Pathway

    DOI: 10.1155/2022/3593294

    PMID: 36238648

    		 Sample: hippocampus