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RNALock Reagent (ref 4992731)

RNALock Reagent (ref 4992731)

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  • Storage

    Store at room temperature (15-25℃ )


  • Description

    The RNALock Reagent is a liquid-form, non-toxic blood preservation reagent. It immediately stabilizes RNA in fresh blood. Human blood samples containing the reagent can be stored at 2-8℃ for 5 days, or at -20℃ for at least three months. Mammal blood samples containing the reagent can be store at 15-25℃ for 2 days, 2-8℃ for 7 days, or at -20℃ for at least six months. Under these conditions, RNA will not show significant degradation. RNAprep Pure Blood Kit with an optimized protocol can ensure rapid extraction of total RNA from blood stored in the RNALock Reagent.


  • Features

    ■ Efficient protection: Protect RNA in fresh blood from degradation.

    ■ Convenient: The operation takes only 2 steps and is suitable for storage of large amount of blood samples.


  • Experimental Procedure



    • Material: 100 μl fresh mouse blood

      Method: The total RNA of fresh mouse blood (stored in RNALock Reagent) that was stored at 15-25℃ , 4-8℃ , -20/-70°C were isolated using the RNAprep Pure Blood Kit (4992238) following the RNALock protocol.

      Results: Please see the above agarose gel electrophoresis picture. 4-6 μl of 50 μl eluates were loaded per lane.

      M: TIANGEN DNA Marker III;

      Lane 1-2 (positive control): RNA purified from fresh blood;

      Lane 3-4: RNA purified from blood samples stored at 15-25℃ for 2 days.

      Lane 5-6: RNA purified from blood samples stored at 4-8℃ for one week.

      Lane 7-8: RNA purified from blood samples stored at -20℃ or -70℃ for half a year.

      The electrophoresis was conducted at 6 V/cm for 30 min on a 1% agarose.

       

      Publications

      ■ Xiao H, Fan X, Zhang R, Wu G. Upregulated N6-Methyladenosine RNA in Peripheral Blood: Potential Diagnostic Biomarker for Breast Cancer. Cancer Res Treat. 2021;53(2):399-408.

       


      ■ Wang Sen, Wan Weijian, Wang Zhuojun, Zhang Huidan, Liu Huan, Arunakumara K. K. I. U., Cui Qiu, Song Xiaojin. A Two-Stage Adaptive Laboratory Evolution Strategy to Enhance Docosahexaenoic Acid Synthesis in Oleaginous Thraustochytrid. Frontiers in Nutrition. 2021