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RNAprep Pure Cell/Bacteria Kit (ref 4992235)

RNAprep Pure Cell/Bacteria Kit (ref 4992235)

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  • Storage

    DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃

    Other reagents could be stored at room temperature (15-25℃)

  • Description

    The RNAprep Pure Cell/Bacteria Kit provides a fast, simple and cost-effective method for purification of total RNA from cultured cells and bacteria samples by using effective spin column and unique buffer system. The kit includes RNase-Free Spin Column CR3 for purifying high quality RNA by using silica-membrane technology. High-quality total RNA could be obtained in 30-40 minutes with high-purity and is free of protein and genomic DNA contamination.

  • Required Reagents

    β-mercaptoethanol, ethanol, lysozyme (optional for bacterial RNA extraction)

  • Features

    ■ Optimized buffers and protocols for cultured cells and bacteria samples make the process simple and convenient.

    ■ Unique DNase I minimizes genomic DNA contamination.

    ■ Unique RNase-Free Filtration Columns CS eliminates other contaminations.

    ■ The high-purity and ready-to-use RNA is suitable for sensitive downstream applications.

    ■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradient centrifugation are needed, which makes the process safe and reliable.

  • Applications

    ■ RT-PCR.

    ■ Northern Blot, Dot Blot.

    ■ Real-Time PCR.

    ■ Chip analysis.

    ■ PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.

Experimental Example

 

  • Material: Human Jurkat Cells (1×106 )

    Method: The total RNA of Human Jukat Cells was isolated using the RNAprep Pure Cell/Bacteria Kit.

    Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 50 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.

     

    • Material: TOP10 E.coli (1×108)

      Method: The total RNA of TOP10 E.coli was isolated using the RNAprep Pure Cell/Bacteria Kit.

      Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 50 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.

       

      Publications

      ■ Liu Y, Cao Z, Wang Y, Guo Y, Xu P, Yuan P, Liu Z, He Y, Wei W. Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol. 2018 Nov 5. 

       

      ■ Qu L, Yi Z, Zhu S, Wang C, Cao Z, Zhou Z, Yuan P, Yu Y, Tian F, Liu Z, Bao Y, Zhao Y, Wei W. Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs. Nat Biotechnol. 2019 Sep;37(9):1059-1069. doi: 10.1038/s41587-019-0178-z. Epub 2019 Jul 15. Erratum in: Nat Biotechnol. 2019 Sep 25;: 

       

      ■ Du L, Lin L, Li Q, Liu K, Huang Y, Wang X, Cao K, Chen X, Cao W, Li F, Shao C, Wang Y, Shi Y. IGF-2 Preprograms Maturing Macrophages to Acquire Oxidative Phosphorylation-Dependent Anti-inflammatory Properties. Cell Metab. 2019 Jun 4;29(6):1363-1375.e8.

       

      ■ Liang Q, Liu Z, Zhu C, Wang B, Liu X, Yang Y, Lv X, Mu H, Wang K. Intrahepatic T-Cell Receptor β Immune Repertoire Is Essential for Liver Regeneration. Hepatology. 2018 Nov;68(5):1977-1990.

       

      ■ Zhang M, Gao C, Guo X, Guo S, Kang Z, Xiao D, Yan J, Tao F, Zhang W, Dong W, Liu P, Yang C, Ma C, Xu P. Increased glutarate production by blocking the glutaryl-CoA dehydrogenation pathway and a catabolic pathway involving L-2-hydroxyglutarate. Nat Commun. 2018 May 29;9(1):2114.

       

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