RNAprep Pure Micro Kit (ref 4992859)
DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃
Other reagents could be store at room temperature (15-25℃)
The function of carrier RNA: A special carrier RNA is provided in the kit. When RNA is extracted from a very small amount of sample, it is recommended to add Carrier RNA in the system, which helps to improve the binding of RNA to adsorption column.
The RNAprep Pure Micro Kit uses a highly efficient, nucleic acid-specific centrifugal adsorption column and a unique buffer system to rapidly extract total RNA from a wide range of different types of microsamples. This kit includes Carrier RNA, which can easily capture trace nucleic acids from the system. The extraction of RNA by this kit is convenient, rapid and reproducible with high yield. The reaction can be completed within 30-40 minutes. The kit can selectively remove all RNA that is 200 nt. The extracted total RNA is extremely pure and free of DNA and protein contamination.
Required Reagentsβ-mercaptoethanol, ethanol
■ Capable of purifying high-quality RNA from trace amount samples, such as micro-dissected tissue, fibrous tissue and cells.
■ Unique DNase I minimizes genomic DNA contamination.
■ The high-purity and ready-to-use RNA is suitable for sensitive downstream applications.
■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradient centrifugation are needed, which makes the process safe and reliable.
■ Northern Blot, Dot Blot.
■ Real-Time PCR.
■ Chip analysis.
■ PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.
Total RNA of 1×106, 1×105, 1×104, 1×103, 1×102, 10 Hela cells were extracted using RNAprep Pure Micro Kit. RT-qPCR was performed using Quant qRT-PCR (SYBR Green) Kit of TIANGEN.
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