RNAprep Pure Plant Kit (ref 4992237)
DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃.
Other reagents could be stored at room temperature (15-25℃)
DescriptionThe RNAprep Pure Plant Kit provides a fast, simple, and cost-effective method for purification of total RNA from plant samples by using effective spin column and unique buffer system. The kit includes RNase-Free Filtration Column CS for homogenizing and filtering viscous plant or fungal lysates, and spin column CR3 for purifying high-quality RNA by using silica-membrane technology. High-quality total RNA could be obtained in 30-40 minutes. The whole process is simple, easy and safe to operate with low toxicity. The obtained RNA has high purity and is free from protein contamination.
Required Reagentsβ-mercaptoethanol, ethanol
■ Optimized buffers for plant samples make the process more convenient.
■ Unique DNase I minimizes genomic DNA contamination.
■ Unique filtration column CS eliminates other contaminations.
■ The high-purity ready-to-use RNA is suitable for sensitive downstream applications.
■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, and no CsCl gradients centrifugation are needed, which makes the process safe and reliable.
■ Northern Blot, Dot Blot.
■ Real-Time PCR.
■ Chip analysis.
■ PolyA Screening, in vitro translation, molecular cloning.
Material: 80 mg Atenia cordifolia leaves
Method: The total RNA of Atenia cordifolia leaves was isolated using the RNAprep Pure Plant Kit.
Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 100 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.
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