Serum / Plasma Circulating DNA Kit (ref 4992289)
StorageRoom temperature (15-25℃)
The Serum/Plasma Circulating DNA Kit is based on silica membrane technology and provides special buffer system. Genomic DNA binds to the silica-membrane in the presence of high salt, while the contaminants pass through the column. After the membrane is thoroughly washed to remove all remaining contaminants, the pure DNA is eluted from the membrane with low salt buffer. The purified DNA can be directly used in downstream molecular biology experiments such as PCR and real time qPCR, etc.
Required ReagentsEthanol, RNaseA (optional)
■ The kit can extract circulating DNA simply and fast by adopting the silica membrane spin column.
■ Safe and non-toxic because there’s no need for phenol/chloroform extraction.
■ Carrier RNA enhances binding of low abundance DNA to spin column membrane.
■ Complete removal of contaminants and PCR inhibitors for downstream experiments.
ApplicationsPCR and real-time qPCR
DNA purified from 400 μl plasma samples using Serum/Plasma Circulating DNA Kit. The following are results of Agilent 2100 analysis after library construction.
1. The circulating DNA purified by Serum/Plasma Circulating DNA Kit is of high purity, and no nonspecific fragments have been detected.
2. The circulating DNA has high recovery rate.
■ Yin JX, Hu WW, Gu H, Fang JM. Combined assay of Circulating Tumor DNA and Protein Biomarkers for early noninvasive detection and prognosis of Non-Small Cell Lung Cancer. J Cancer. 2021 Jan 1;12(4):1258-1269.