Superoxide Dismutase T-SOD Assay Kit WST-1 method - E-BC-K020-M

Superoxide Dismutase T-SOD Assay Kit WST-1 method - E-BC-K020-M

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Detection principle

The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.


Performance characteristics

Synonyms T-SOD
Sample type Serum,plasma,hydrothorax,ascites,urine,cells,tissue
Sensitivity 0.2 U/mL
Detection range 0.2 -14.4 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 30 min
Precision Average inter-assay CV: 3.700%Average intra-assay CV: 2.900%
Other instruments required Micropipettor, Multichannel pipettor, Vortex mixer, Incubator
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage Reagent 3: -20℃, others: 2-8℃
Valid period 12 months


The activity of SOD increased at the beginning of the UV exposure until 80 mJ/cm2, then decreased with the UV fluence reached 120 mJ/cm2. The induced SOD activity was higher in the UV/Cl2 process (purple) compared with that of the UV alone process.

Q Wan et al investigate the efficiency and mechanism of fungal spore disinfection using UV-LEDs irradiation and chlorine. Total superoxide dismutase (T-SOD) activity of fungal spore was determined using T-SOD activity assay kit (E-BC-K020-M).

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 25%~65% (the optimal inhibition ratio is the range of 40%~60%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Rat serum




Human hydrothorax


Cell culture supernatant


10% Rat liver tissue homogenate


10% Rat heart tissue homogenate


10% Rat kidney tissue homogenate


10% Rat brain tissue homogenate


10% Plant tissue homogenate


HepG2 cells (3 mgprot/mL)


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


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