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T-SOD Assay Kit Hydroxylamine method - E-BC-K019-S

T-SOD Assay Kit Hydroxylamine method - E-BC-K019-S

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Detection principle

The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.

 

Performance characteristics

Synonyms T-SOD
Sample type Serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity 4.7 U/mL
Detection range 4.7-166 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 100 min
Precision Average inter-assay CV: 6.300%Average intra-assay CV: 2.800%
Other instruments required Micropipettor, Vortex mixer, Water bath, Centrifuge
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage Reagent 4: -20℃, others: 2-8℃
Valid period 12 months

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

The volume of sample

HepG2 supernatant

1

50 μL

HepG2 cell

8-10

25 μL

Mouse serum

3-5

20 μL

10% Mouse liver tissue homogenate

40-60

20 μL

10% Rat kidney tissue homogenate

15-20

20 μL

Human urine

1

25 μL

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


Citations


  1. Bioactive Materials (2023) IF: 16.874
    Gold nanoparticles-embedded ceria with enhanced antioxidant activities for treating inflammatory bowel disease

    DOI: 10.1016/j.bioactmat.2023.01.015
    Sample: colon tissue
  2. JOURNAL OF HAZARDOUS MATERIALS (2021) IF: 10.588
    Sequential use of UV-LEDs irradiation and chlorine to disinfect waterborne fungal spores: Efficiency, mechanism and photoreactivation

    DOI: 10.1016/j.jhazmat.2021.127102

    PMID: 34482083