TGuide Smart Magnetic Plant RNA Kit - 4993552
TGuide Smart Magnetic Plant RNA Kit
Purify high-quality total RNA from plant tissues
Catalog number / packaging
No. of preps
•Purify high-yield, high-purity, high-quality total RNA from plant tissues.
•No toxic reagents such as phenol/chloroform contained.
•The RNA obtained has high purity and can be directly used for chip detection, high-throughput sequencing and other experiments.
Super easy to use.
No extra pipetting work.
The elution volume can be adjusted as required.
The installation and operation require little training. With pre-installed programs, unpack the cartridge, select the protocol and run your experiment. Programs are ready-to-use, and also customizable.
Preloaded reagents and matched disposable consumables.The chemical handling is eliminated to the greatest extent to ensure the operation safety.
Wheat root total RNA extraction
Sample size: 100 mg
Sample pretreatment: grinding with liquid nitrogen or low temperature tissue homogenizer treatment
Agarose gel concentration :2%(TAE)
Loading volume: 2 μl
Marker: Marker IV, TIANGEN
Experiment result: using TGuide S16 achieved with reagent 4993552 to automatically extract the total RNA of wheat roots has good yield and high purity. In this experiment, about 20 μg of nucleic acid was extracted from 100 mg wheat roots, with OD260/OD280 around 2.0~2.1, and OD260/OD230＞2.0.
Total RNA extraction from different plant samples
Sample size: 100 mg
Sample pretreatment: low temperature homogenizer
Agarose gel concentration :1% (TAE)
Loading volume: 1 μl
M: Marker III, TIANGEN
1-4: corn seeds 5-8: corn leaves 9-12: cabbage roots 13-16: soybeans
The first two samples were extracted by spin-column extraction kit and the last two samples were extracted by TGuide S16.
Experiment result: the extraction yield of plant RNA by TGuide S16 with reagent 4993552 is equivalent to or better than that by the spin-column extraction kit. The purity
and integrity are basically the same. Thus, TGuide S16 can be used to extract plant total RNA in replacement of the spin-column based extraction solution.