TIANamp Bacteria DNA Kit (ref 4992448)
StorageRoom temperature (15-25℃)
DescriptionThe TIANamp Bacteria DNA Kit adopts a highly efficient, DNA-specific adsorption spin column and a unique buffer system for both genomic DNA extraction from Gram-negative and Gram-positive bacteria. The kit can also be used for the extraction of pathogenic bacteria (microorganisms) of food, such as Staphylococcus aureus, cholera sclerotia, hemorrhagic Escherichia coli O157:H7, Listeria monocytogenes, Salmonella, Enterobacter sakazakii, etc. 10-40 μl of pure genomic DNA can be rapidly extracted and purified from 1-5 ml of bacterial culture medium, and the obtained genomic DNA can be directly used in molecular biology experiments such as PCR template, restriction enzyme digestion and Southern blot.
Required ReagentsLysozyme (Gram-positive bacteria), ethanol, lysozyme buffer (20 mM Tris, pH8.0; 2 mM Na2-EDTA, 1.2% Triton-100)
■ Simple and fast: High purity genomic DNA of Gram-negative bacteria can be obtained within 1 hour
■ Excellent quality: The purified DNA can be directly used in downstream molecular experiments such as PCR, restriction endonuclease digestion, Southern blotting, etc.
■ Genome extraction of Gram-negative bacteria
■ Genome extraction of Gram-positive bacteria
■ Genome extraction of pathogenic bacteria in food
DNA Extraction Yield From Various Tissues:
Note: The DNA extraction amount may vary depending on the bacteria types and culture time, etc. Gram positive bacteria require special treatments such as lysozyme for lysing, and the genomic DNA extraction can be performed according to the procedures of Gram-negative bacteria.
Extraction of bacterial genomic DNA from various sources using TIANamp Bacteria DNA Kit.
Starting amount: 1 ml of overnight bacteria culture medium. Elution volume: 100 μl. Loading volume: 3 μl. The concentration of the agarose gel was 2%. The electrophoresis was performed under 6V/cm for 20 min.
M: λDNA/Hind III Marker;
3: Staphylococcus epidermidis;
4: Staphylococcus aureus.
Publications■ Liu, W., Cremer, J., Li, D., Hwa, T. & Liu, C. An evolutionarily stable strategy to colonize spatially extended. 240 habitats. Nature 575, 664–668 (2019)■ Sun J, Chen C, Cui CY, Zhang Y, Liu X, Cui ZH, Ma XY, Feng Y, Fang LX, Lian XL, Zhang RM, Tang YZ, Zhang KX, Liu HM, Zhuang ZH, Zhou SD, Lv JN, Du H, Huang B, Yu FY, Mathema B, Kreiswirth BN, Liao XP, Chen L, Liu YH. Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli. Nat Microbiol. 2019 Sep;4(9):1457-1464.