TIANamp Genomic DNA Kit (ref 4992199)
StorageRoom temperature (15-25℃)
DescriptionThe TIANamp Genomic DNA Kit provides a fast and easy silica-based format for DNA purification. The kit simplifies the purification of DNA from a wide range of sample types, including animal blood (≤ 1 ml), cultured cells, and tissues commonly encountered in life science, veterinary, and genotyping applications. Most samples could be directly lysed with the provided buffer system, eliminating the need for mechanical disruption and also reducing hands-on time. The kit purifies high-quality DNA without proteins and inhibitors. The purified DNA is suitable for PCR, restriction digestion and Southern blot.
Required ReagentsRed Cell Lysis Buffer, ethanol, RNase A (optional)
■ Silica-membrane technology provides fast way to purify ready-to-use high quality gDNA.
■ High DNA yield, excellent repeatability.
■ This kit significantly removes contaminants and inhibitors, facilitating downstream applications.
■ Please use fresh samples to ensure the integrity of the purified gDNA.
■ If the sample starting amount exceeds the recommended range of the kit, it is recommended to increase the amount of Buffer GA and Buffer GB, and split the column loading in separate times, or perform the experiment using two spin columns.
■ For long-term storage of the gDNA, it is recommended to store in buffer TE. Please ensure that the OD260/OD280 value is in the range of 1.7-1.9, and the concentration of gDNA is 100-300 ng/μl.
DNA Extraction Yield From Various Tissues
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■ Xia P, Wang S, Ye B, Du Y, Li C, Xiong Z, Qu Y, Fan Z. A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion. Immunity. 2018 Apr 17;48(4):688-701.e7.
■ Zhang H, Tang K, Ma J, Zhou L, Liu J, Zeng L, Zhu L, Xu P, Chen J, Wei K, Liang X, Lv J, Xie J, Liu Y, Wan Y, Huang B. Ketogenesis-generated β-hydroxybutyrate is an epigenetic regulator of CD8+ T-cell memory development. Nat Cell Biol. 2020 Jan;22(1):18-25.
■ Xiao CL, Zhu S, He M, Chen D, Zhang Q, Chen Y, Yu G, Liu J, Xie SQ, Luo F, Liang Z, Wang DP, Bo XC, Gu XF, Wang K, Yan GR. N6-Methyladenine DNA Modification in the Human Genome. Mol Cell. 2018 Jul 19;71(2):306-318.e7.
■ Sheng C, Yao C, Wang Z, Chen H, Zhao Y, Xu D, Huang H, Huang W, Chen S. Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing. Nat Commun. 2018 Oct 22;9(1):4381.
■ Xie J, Ge W, Li N, Liu Q, Chen F, Yang X, Huang X, Ouyang Z, Zhang Q, Zhao Y, Liu Z, Gou S, Wu H, Lai C, Fan N, Jin Q, Shi H, Liang Y, Lan T, Quan L, Li X, Wang K, Lai L. Efficient base editing for multiple genes and loci in pigs using base editors. Nat Commun. 2019 Jun 28;10(1):2852.