TIANamp Stool DNA Kit is based on silica membrane technology for stool sample gDNA extraction. The spin column is made of new type silica membrane which can bind DNA optimally on given salt and pH conditions. Simple centrifugation processing completely removes impurity, proteins and other organic compound. The gDNA isolated with these products is of high quality, high purity and full-length. Purified gDNA is ready for use in downstream applications such as PCR.

■ Wide range of applications: Applicable for solid or liquid stool samples from different sources.

■ Simple and fast: Ultra-pure gDNA can be obtained within 1 hour.

■ High purity: High efficiency precipitant can remove impurities such as humic acid, the purity of extracted DNA is very high, which can be directly used in downstream experiments.

Applicable for solid or liquid stool samples from different sources

■ Repeated freezing and thawing should be avoided, otherwise it will result in small fragment DNA and decrease of yield.

■ If precipitates formed in Buffer SA or Buffer SC, warm buffer to 37°C until precipitates fully dissolve.

■ If the sample absorbs much water, the amount of Buffer SA and Buffer SC can be increased in equal proportion. If Buffer SA and SC are insufficient, they can be purchased separately.

Publications

■ Chen K, Luan X, Liu Q, Wang J, Chang X, Snijders AM, Mao JH, Secombe J, Dan Z, Chen JH, Wang Z, Dong X, Qiu C, Chang X, Zhang D, Celniker SE, Liu X. Drosophila Histone Demethylase KDM5 Regulates Social Behavior through Immune Control and Gut Microbiota Maintenance. Cell Host Microbe. 2019 Apr 10;25(4):537-552.e8. 

■ Li B, Li L, Li M, Lam SM, Wang G, Wu Y, Zhang H, Niu C, Zhang X, Liu X, Hambly C, Jin W, Shui G, Speakman JR. Microbiota Depletion Impairs Thermogenesis of Brown Adipose Tissue and Browning of White Adipose Tissue. Cell Rep. 2019 Mar 5;26(10):2720-2737.e5