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Total Oxidant Status TOS Colorimetric Assay Kit - E-BC-K802-M

Total Oxidant Status TOS Colorimetric Assay Kit - E-BC-K802-M

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Detection principle

Under acid conditions, the oxidizing material in the sample can oxidize Fe2+ to Fe3+, which binds highly with xylenol orange to produce a blue-purple complex. When the pH of solution is in the range of 2-3, its maximum absorption wavelength is around 590 nm, and the color depth is proportional to the content of oxidation substances in a certain concentration and a certain time, so as to indirectly calculate the total oxidation state of the sample.

Performance characteristics

Synonyms TOS
Sample type cell, tissue, serum and other liquid samples
Sensitivity 2.5 μmol H2O2 Equiv./L
Detection range 2.5-100 μmol H2O2 Equiv./L
Detection method Colorimetric method
Assay type Quantitative
Assay time 30 min
Precision Average inter-assay CV: 3.500%Average intra-assay CV: 2.300%
Other instruments required Micropipettor, 37℃ incubator.
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

 

Different subscript letters indicate significant differences among the groups. (P<0.05)

X Zhao et al investigate the function of quercetin for reducing ethanol-induced hepatocyte pyroptosis. Total oxidants status (TOS) of human L02 cell was determined using TOS colorimetric assay kit (E-BC-K802-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2.5-100 μmol H2O2 Equiv./L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Porcine serum

1

Horse serum

1

Cynomolgus macaques serum

1

10% Rat liver tissue homogenate

1

10% Rat kidney tissue homogenate

1

10% Rat lung tissue homogenate

1

10% Rat spleen tissue homogenate

1

0.612×10^6 Molt-4 cells

1


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).



Citations


  1. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Quercetin Protects Ethanol-Induced Hepatocyte Pyroptosis via Scavenging Mitochondrial ROS and Promoting PGC-1α-Regulated Mitochondrial Homeostasis in L02 Cells

    DOI: 10.1155/2022/4591134
    Sample: L02 cell
  2. Biology-Basel (2021) IF: 5.079
    Cellular Stress Pathways Are Linked to Acetamiprid-Induced Apoptosis in SH-SY5Y Neural Cells

    DOI: 10.3390/biology10090820
    Sample: SH-SY5Y cell