Vitamin E VE Assay Kit - E-BC-K033-S

Vitamin E VE Assay Kit - E-BC-K033-S

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Detection principle

Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. VE content can be calculated by measuring the OD value at 532 nm.

Performance characteristics

Synonyms VE
Sample type Serum,plasma,tissue
Sensitivity 0.09 μg/mL
Detection range 0.09-40 μg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 60 min
Precision Average inter-assay CV: 4.900%Average intra-assay CV: 4.400%
Other instruments required Micropipettor, Vortex mixer, Centrifuge
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol,N-heptane
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.09-40 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Human plasma


10% Mouse liver tissue homogenization


Rat serum


Rat plasma


10% Carrot tissue homogenization


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets
  1. DOI: 10.3390/nu15040832

    Sample: serum
  2. LIFE SCIENCES (2020) IF: 3.647
    Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis

    DOI: 10.1016/j.lfs.2020.117584

    PMID: 32220623

    Sample: Tissue homogenate