Vitamin E VE Assay Kit - E-BC-K033-S
Detection principle
Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. VE content can be calculated by measuring the OD value at 532 nm.
Performance characteristics
| Synonyms | VE |
| Sample type | Serum,plasma,tissue |
| Sensitivity | 0.09 μg/mL |
| Detection range | 0.09-40 μg/mL |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 60 min |
| Precision | Average inter-assay CV: 4.900%Average intra-assay CV: 4.400% |
| Other instruments required | Micropipettor, Vortex mixer, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol,N-heptane |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.09-40 μg/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
Human serum |
1 |
|
Human plasma |
1 |
|
10% Mouse liver tissue homogenization |
1 |
|
Rat serum |
1 |
|
Rat plasma |
1 |
|
10% Carrot tissue homogenization |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets-
DOI: 10.3390/nu15040832
Sample: serum -
LIFE SCIENCES (2020) IF: 3.647
Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesisPMID: 32220623
Sample: Tissue homogenate